TextbookInfectious DiseasesFever of Unknown Origin

Fever of Unknown Origin

Defined as fever >38.3°C on several occasions, lasting >3 weeks, with no diagnosis despite 1 week of inpatient investigation (Petersdorf & Beeson criteria). The three main categories are infection (~30%), malignancy (~20%), and autoimmune/inflammatory conditions (~20%). Approximately 10–15% remain undiagnosed despite thorough investigation.

Key Facts

Classic definition: fever >38.3°C on several occasions, duration >3 weeks, no diagnosis after 1 week of investigation Infections (~30%): TB, endocarditis, abscess, osteomyelitis, CMV, EBV, HIV Malignancy (~20%): lymphoma (most common), renal cell carcinoma, hepatocellular carcinoma, leukaemia Autoimmune/inflammatory (~20%): adult-onset Still's disease, SLE, GCA/PMR, vasculitis, sarcoidosis Adult-onset Still's disease: quotidian (daily spiking) fever, salmon-pink evanescent rash, arthralgia, ferritin markedly elevated (>10,000 μg/L) PET-CT: increasingly used to identify occult infection or malignancy — sensitivity ~85–90% for FUO Undiagnosed FUO: ~10–15% remain without diagnosis; most have benign course and fever resolves Drug fever: consider in all FUO cases — temporal correlation with drug initiation; resolves on withdrawal

Overview

Key Facts

FUO requires a systematic and thorough approach. The differential is vast but can be narrowed by careful history, examination, and staged investigations.

Epidemiology

  • Infections: declining proportion due to improved diagnostics; TB remains important
  • Malignancy: lymphoma is the single most common malignant cause
  • Autoimmune: increasingly recognised with better diagnostics
  • Drug fever: underdiagnosed cause in hospitalised patients

Aetiology

Infectious (~30%): TB, endocarditis, abscess (intra-abdominal, pelvic, dental), osteomyelitis, CMV, EBV, HIV, Q fever, brucellosis Malignant (~20%): lymphoma, leukaemia, renal cell carcinoma, HCC, atrial myxoma Autoimmune (~20%): adult-onset Still's, SLE, GCA/PMR, PAN, sarcoidosis, Crohn's Miscellaneous (~15%): drug fever, factitious fever, thyrotoxicosis, adrenal insufficiency, PE, familial Mediterranean fever

Pathophysiology

  • Fever: regulated by hypothalamic set-point elevation via prostaglandin E2 (PGE2)
  • Pyrogens: exogenous (microbial products) and endogenous (IL-1, IL-6, TNF-alpha)
  • Multiple pathological processes can sustain chronic febrile response

Clinical Presentation

Key History

  • Fever pattern: quotidian (daily spike — Still's), tertian/quartan (malaria), Pel-Ebstein (Hodgkin's lymphoma — rare)
  • Travel history, animal/occupational exposure
  • Medication list (drug fever)
  • Family history (familial Mediterranean fever)
  • Immunosuppression, HIV risk factors

Key Examination

  • Lymphadenopathy (lymphoma, TB, SLE)
  • Heart murmur (endocarditis)
  • Hepatosplenomegaly (haematological malignancy, infection)
  • Dental examination (dental abscess)
  • Temporal artery tenderness (GCA)
  • Skin rash (Still's, vasculitis, SLE)

Red Flags

  • Weight loss >10% (malignancy, TB)
  • Night sweats (lymphoma, TB, endocarditis)
  • New heart murmur (endocarditis)
  • Neurological symptoms (CNS lymphoma, TB meningitis)

Differential Diagnosis

DiagnosisKey FeaturesInvestigation
TBWeight loss, night sweats, lymphadenopathy, immigration historyCXR, sputum AFB, IGRA
EndocarditisNew murmur, splinter haemorrhages, embolic phenomenaBlood cultures ×3, TOE
LymphomaLymphadenopathy, B-symptoms, hepatosplenomegalyCT TAP, lymph node biopsy
Adult-onset Still'sQuotidian fever, evanescent rash, arthralgia, ferritin >10,000Ferritin, FBC, LFTs
GCA/PMRAge >50, headache, jaw claudication, proximal myalgiaESR/CRP, temporal artery biopsy
Drug feverTemporal relationship with drug, eosinophiliaDrug history, withdrawal trial

Diagnosis / Investigation

First-Line

  • FBC with differential: leucocytosis, lymphopenia, eosinophilia, cytopenias
  • CRP/ESR: degree of inflammation
  • LFTs, U&Es, LDH: organ involvement
  • Blood cultures: minimum 3 sets from different sites
  • Urinalysis and urine culture
  • CXR: TB, lymphoma, sarcoidosis
  • HIV test: always include

Second-Line

  • CT thorax/abdomen/pelvis: abscess, lymphadenopathy, solid organ malignancy
  • Autoimmune screen: ANA, ANCA, RF, complement, ferritin
  • Serology: CMV, EBV, hepatitis, Q fever, Brucella, Bartonella
  • IGRA or Mantoux: TB
  • TTE/TOE: endocarditis
  • Peripheral blood film: haematological malignancy, haemophagocytosis

Third-Line

  • PET-CT: sensitivity ~85–90% for identifying source in FUO — increasingly first choice
  • Bone marrow biopsy: lymphoma, granulomatous disease, haemophagocytic lymphohistiocytosis
  • Temporal artery biopsy: if GCA suspected (age >50, raised ESR)
  • Liver biopsy: granulomatous hepatitis
  • Lymph node biopsy: if lymphadenopathy

Management

General Approach

  • Avoid empirical antibiotics unless clinically deteriorating — they obscure diagnosis
  • Systematic investigation: staged approach (first, second, third-line)
  • Review medication list: stop non-essential drugs to exclude drug fever
  • Repeat examination: daily — new signs may emerge

Specific Treatment

  • Treat underlying cause once identified
  • TB: standard quadruple therapy (RIPE)
  • Endocarditis: prolonged IV antibiotics
  • Lymphoma: oncology referral for chemotherapy
  • Adult-onset Still's: NSAIDs, corticosteroids (prednisolone 0.5–1mg/kg), IL-1 inhibitors (anakinra) if refractory
  • GCA: prednisolone 40–60mg OD (start immediately if visual symptoms)
  • Drug fever: withdraw causative drug — fever typically resolves within 48–72 hours

Naproxen Test

  • Naproxen 250mg BD for 3 days
  • Historically suggested: fever resolution suggests neoplastic cause (Naprosyn test) — limited evidence but still referenced

Referral Criteria

  • Infectious diseases: complex cases, unexplained FUO
  • Haematology: suspected lymphoma/leukaemia
  • Rheumatology: suspected autoimmune cause
  • Nuclear medicine: PET-CT for unexplained FUO

Prognosis

  • ~85–90% of FUO cases eventually diagnosed with thorough investigation
  • 10–15% remain undiagnosed — majority have benign course with spontaneous resolution
  • Prognosis depends entirely on underlying cause
  • Undiagnosed FUO: 5-year mortality ~3% (generally favourable)
  • Infection-related FUO: good prognosis with appropriate treatment
  • Malignancy-related FUO: variable depending on type and stage

Other Relevant Information

Categories of FUO

CategoryDefinitionCommon Causes
ClassicFever >3 weeks, no diagnosis after 1 week inpatientTB, lymphoma, Still's, endocarditis
NosocomialHospitalised ≥24h, fever develops after admissionLine infection, C. difficile, drug fever, PE
NeutropenicNeutrophils <0.5 ×10⁹/L, fever >3 daysOpportunistic fungi, resistant bacteria
HIV-associatedHIV+, fever >4 weeks (outpatient) or >3 days (inpatient)TB, MAC, CMV, lymphoma, PCP

FUO Investigation Yield

InvestigationDiagnostic Yield
Blood cultures5–10%
CT TAP15–20%
PET-CT50–70%
Bone marrow biopsy5–15%
Temporal artery biopsyVariable (if indicated)