TextbookHaematologyAcute Lymphoblastic Leukaemia

Acute Lymphoblastic Leukaemia

Malignant proliferation of lymphoid blast cells in bone marrow and blood. Most common childhood cancer (peak age 2–5 years). Highly curable in children (~90% survival) but carries a poorer prognosis in adults.

Key Facts

Most common childhood cancer: peak incidence 2–5 years; ~85% are B-cell ALL Bone marrow failure: anaemia (pallor, fatigue), neutropenia (infections), thrombocytopenia (bleeding, bruising) Lymphoblasts ≥20% in bone marrow (WHO definition) Philadelphia chromosome t(9;22): BCR-ABL1 fusion; present in ~25% of adult ALL (poor prognosis; add TKI — imatinib) CNS involvement: prophylactic intrathecal methotrexate + cranial radiation (children) to prevent CNS relapse Treatment (children): induction (dexamethasone, vincristine, asparaginase, daunorubicin) → consolidation → maintenance (2–3 years) Child survival: ~90% cure rate; adult ALL ~40–50% 5-year survival Tumour lysis syndrome: major risk during initial chemotherapy; prevent with allopurinol/rasburicase, aggressive hydration

Overview

Key Facts

Acute lymphoblastic leukaemia (ALL) is a malignancy of lymphoid progenitor cells (lymphoblasts) that accumulate in the bone marrow, blood, and extramedullary sites.

Epidemiology

  • Most common childhood cancer: ~400 new cases/year in UK children
  • Peak incidence: 2–5 years (B-cell ALL)
  • Second peak: >50 years (adult ALL; poorer prognosis)
  • Slight male predominance

Aetiology

  • Mostly unknown; likely multistep oncogenesis
  • Risk factors: Down syndrome (20× risk), radiation, previous chemotherapy
  • Genetic: ETV6-RUNX1 t(12;21) — good prognosis; Philadelphia chromosome t(9;22) — poor (adult)

Pathophysiology

  • Malignant transformation of lymphoid precursors in bone marrow
  • Clonal expansion of lymphoblasts → marrow failure (normal haematopoiesis suppressed)
  • Extramedullary infiltration: liver, spleen, lymph nodes, CNS, testes
  • B-cell ALL (~85% children), T-cell ALL (~15%)

Clinical Presentation

Typical Presentation

  • Bone marrow failure: anaemia (pallor, fatigue), infections (neutropenia), bleeding/bruising (thrombocytopenia)
  • Bone pain: especially in children (marrow expansion)
  • Hepatosplenomegaly and lymphadenopathy
  • Constitutional: fever, weight loss, night sweats

Specific

  • Mediastinal mass: T-cell ALL (thymic involvement → SVC obstruction)
  • CNS: headache, cranial nerve palsies, vomiting (leptomeningeal infiltration)
  • Testicular enlargement: boys; sanctuary site

Red Flags

  • Child with pallor, bruising, bone pain, hepatosplenomegaly
  • Mediastinal mass with respiratory compromise
  • CNS symptoms
  • Tumour lysis syndrome at presentation or early treatment

Differential Diagnosis

DiagnosisKey FeaturesInvestigation
AMLMyeloid blasts, Auer rods, MPO positiveBone marrow, flow cytometry
Aplastic anaemiaPancytopenia, hypocellular marrow, no blastsBone marrow
Infectious mononucleosisReactive lymphocytes, pharyngitis, hepatosplenomegalyMonospot, EBV serology
JIA (systemic)Fever, rash, arthritis, no blasts on marrowFBC, bone marrow
NeuroblastomaYoung child, abdominal mass, raised catecholaminesImaging, urine catecholamines

Diagnosis / Investigation

Bloods

  • FBC: anaemia, thrombocytopenia, WCC variable (low/normal/very high)
  • Blood film: circulating lymphoblasts (large cells, high nuclear:cytoplasmic ratio, fine chromatin)
  • LDH, urate: raised (high cell turnover)
  • Coagulation: may be deranged
  • U&Es, calcium, phosphate: tumour lysis syndrome risk assessment

Bone Marrow

  • Aspirate + trephine: ≥20% lymphoblasts (diagnostic)
  • Flow cytometry/immunophenotyping: B-cell (CD10, CD19, CD20) vs T-cell (CD3, CD7) lineage
  • Cytogenetics: t(12;21) ETV6-RUNX1 (good), t(9;22) BCR-ABL (poor), hyperdiploidy (good), hypodiploidy (poor)
  • Molecular: BCR-ABL1, MLL rearrangements, IKZF1

Special Tests

  • LP (CSF): CNS involvement assessment
  • CXR: mediastinal mass (T-cell ALL)
  • Testicular USS: if enlarged (boys)

Management

Childhood ALL (UK UKALL Protocol)

Induction (4–6 weeks):

  • Dexamethasone (or prednisolone) + vincristine + asparaginase + daunorubicin
  • Aim: complete remission (CR) in ~95%

Consolidation:

  • High-dose methotrexate, cyclophosphamide, cytarabine
  • Intrathecal methotrexate (CNS prophylaxis)

Maintenance (2–3 years total):

  • Daily 6-mercaptopurine + weekly methotrexate

CNS-directed therapy:

  • Intrathecal methotrexate/cytarabine/hydrocortisone throughout treatment

Adult ALL

  • Similar intensive chemotherapy protocols
  • Philadelphia positive: add TKI (imatinib, dasatinib) throughout
  • Allogeneic SCT: in first CR for high-risk (Ph+, poor cytogenetics, slow response)

Relapsed/Refractory

  • Blinatumomab (bispecific T-cell engager; anti-CD19/CD3; NICE TA450)
  • Tisagenlecleucel (CAR-T; anti-CD19; NICE TA554): for relapsed/refractory B-cell ALL in children
  • Inotuzumab ozogamicin (anti-CD22-ADC; NICE TA541)
  • Allogeneic SCT: post-reinduction

Supportive

  • Tumour lysis syndrome prevention: allopurinol/rasburicase, IV fluids
  • Infection prevention: PCP prophylaxis (co-trimoxazole), antifungals
  • Blood product support

Referral Criteria

  • Paediatric oncology: all childhood ALL (principal treatment centres)
  • Haematology: adult ALL
  • Urgent: suspected acute leukaemia (same-day blood film)

Prognosis

  • Childhood ALL: ~90% 5-year survival (one of the great successes of modern oncology)
    • Good risk: age 1–9, WCC <50, hyperdiploidy, ETV6-RUNX1 → >95% cure
    • Poor risk: Ph+, infant (<1 year), hypodiploidy, MLL rearrangement
  • Adult ALL: ~40–50% 5-year survival; Ph+ historically ~20% (improved with TKIs to ~60%)
  • CNS relapse: reduced to <5% with intrathecal prophylaxis
  • Late effects in survivors: secondary cancers, cardiac toxicity, neurocognitive effects, infertility, growth impairment

Other Relevant Information

Prognostic Factors

FactorGoodPoor
Age1–9 years<1 or >10 years
WCC<50 × 10⁹/L>50 × 10⁹/L
CytogeneticsHyperdiploidy, t(12;21)Hypodiploidy, t(9;22), MLL
ImmunophenotypePrecursor B-cellT-cell (variable)
MRD (day 29)NegativePositive