TextbookClinical SciencesClinical Trials and Evidence-Based Medicine

Clinical Trials and Evidence-Based Medicine

Clinical trials are structured research studies evaluating interventions in humans. Evidence-based medicine integrates best research evidence with clinical expertise and patient values.

Key Facts

Phase I trials assess safety and dosing in healthy volunteers (20-100 participants); Phase II assess efficacy; Phase III are large RCTs comparing to standard treatment Randomisation minimises confounding and selection bias; allocation concealment prevents foreknowledge of group assignment Double-blinding reduces observer and performance bias — neither participant nor investigator knows allocation Intention-to-treat (ITT) analysis includes all randomised participants regardless of compliance — preserves randomisation Per-protocol analysis includes only those who completed the study as planned — may overestimate treatment effect CONSORT statement provides reporting guidelines for RCTs — includes flow diagram of participant progress Equipoise is the ethical requirement that genuine uncertainty exists about which treatment is superior before conducting an RCT GCP (Good Clinical Practice) guidelines ensure trials are ethically and scientifically sound — required by MHRA

Overview

Key Facts

Clinical trials are the cornerstone of evidence-based medicine, providing the most reliable evidence for treatment efficacy and safety. The UK has a strong clinical trials infrastructure with the NIHR coordinating much of the research activity.

Epidemiology

Approximately 600,000 people participate in clinical trials in the UK each year. The NIHR supports over 3,500 active studies. UK clinical trials legislation is governed by the Medicines for Human Use (Clinical Trials) Regulations 2004, overseen by the MHRA.

Aetiology

Trial phases:

  • Phase I: First-in-human, safety, pharmacokinetics, dose-finding (20-100 healthy volunteers)
  • Phase II: Efficacy and side effects in target population (100-300 patients)
  • Phase III: Large-scale RCT comparing to standard care or placebo (300-3,000+ patients)
  • Phase IV: Post-marketing surveillance, rare side effects, long-term outcomes

Pathophysiology

Key methodological concepts:

  • Randomisation methods: Simple, block, stratified, minimisation
  • Blinding levels: Open-label, single-blind, double-blind, triple-blind
  • Control types: Placebo, active comparator, standard care
  • Endpoints: Primary (main outcome), secondary, surrogate (biomarker proxy), composite
  • Non-inferiority trials: Show new treatment is not unacceptably worse than standard (pre-specified margin)
  • Adaptive designs: Allow modifications based on interim data (e.g., RECOVERY trial for COVID-19 treatments)

Clinical Presentation

Applying Evidence to Clinical Practice

  • Ask: Formulate a clinical question (PICO: Population, Intervention, Comparator, Outcome)
  • Acquire: Search for best available evidence (PubMed, Cochrane, NICE)
  • Appraise: Critically evaluate validity, results, and applicability
  • Apply: Integrate evidence with clinical expertise and patient preferences
  • Assess: Audit outcomes of clinical decisions

Ethical Considerations

  • Informed consent — voluntary, informed, competent
  • Research Ethics Committee (REC) approval required
  • Declaration of Helsinki principles
  • Data Safety Monitoring Board (DSMB) reviews interim data for safety

Red Flags in Trial Interpretation

  • Unblinded trials with subjective outcomes — high risk of performance bias
  • Large loss to follow-up (>20%) — threatens validity
  • Surrogate endpoints without validated link to clinical outcomes
  • Selective outcome reporting — check trial registry (ClinicalTrials.gov) for pre-specified outcomes

Differential Diagnosis

Study DesignStrengthsLimitations
RCTMinimises confounding, highest internal validityExpensive, may lack external validity, ethical constraints
Cohort studyCan study rare exposures, temporal relationshipConfounding, loss to follow-up, expensive if prospective
Case-controlEfficient for rare diseases, quick and cheapRecall bias, cannot calculate incidence/RR directly
Cross-sectionalQuick, cheap, estimates prevalenceCannot determine causation, temporal ambiguity
Systematic review/meta-analysisPools evidence, increases powerPublication bias, heterogeneity, garbage in-garbage out
Qualitative researchExplores experiences and perspectivesNot generalisable, subjective interpretation

Diagnosis / Investigation

Critical Appraisal Framework (CASP)

  • Validity: Was randomisation adequate? Was blinding maintained? Were groups similar at baseline?
  • Results: What is the effect size (RR, OR, NNT)? How precise (confidence intervals)?
  • Applicability: Are patients similar to mine? Are all important outcomes considered? Are benefits worth harms and costs?

Key Statistical Measures

  • Absolute risk reduction (ARR): Difference in event rates between groups
  • Relative risk reduction (RRR): ARR ÷ control event rate
  • Number needed to treat (NNT): 1/ARR
  • Hazard ratio: Instantaneous risk ratio (from Cox regression, survival analysis)
  • Forest plot: Visual display of individual study and pooled results in meta-analysis
  • Funnel plot: Detects publication bias in meta-analyses (asymmetry suggests bias)

Regulatory Framework

  • MHRA approval for investigational medicinal products
  • Health Research Authority (HRA) approval
  • Research Ethics Committee (REC) review
  • Sponsor responsibilities (university, NHS trust, or pharmaceutical company)

Management

Non-pharmacological

  • Shared decision-making using evidence summaries (NICE patient decision aids)
  • Clinical guidelines (NICE, SIGN, RCGP) synthesise evidence for practice
  • Clinical audit measures practice against evidence-based standards

Pharmacological

Landmark UK Trials (examples):

  • RECOVERY trial: Dexamethasone reduces COVID-19 mortality in ventilated patients by 36%
  • UKPDS: Intensive glucose control reduces microvascular complications in T2DM
  • ISIS-2: Aspirin + streptokinase reduces MI mortality
  • 4S trial: Simvastatin reduces CV events in hypercholesterolaemia
  • NICE guidance is based on systematic review of clinical and cost-effectiveness evidence

Referral Criteria

  • Consider referring suitable patients to clinical trials — all NHS trusts should facilitate research participation
  • Research nurse support available through NIHR Clinical Research Network
  • Patient and public involvement (PPI) is now mandatory in NIHR-funded research

Prognosis

  • Evidence-based medicine has improved outcomes across all medical specialties
  • Implementation of trial evidence into guidelines typically takes 17 years on average — a key challenge
  • The RECOVERY trial demonstrated the value of large, simple adaptive platform trials during pandemics
  • Approximately 50% of treatments used in clinical practice have been tested in RCTs
  • UK clinical trials contribute significantly to global medical knowledge through the NIHR infrastructure

Other Relevant Information

Hierarchy of Evidence

LevelStudy TypeStrength
1aSystematic review of RCTsHighest
1bIndividual RCT with narrow CIHigh
2aSystematic review of cohort studiesModerate-high
2bIndividual cohort studyModerate
3Case-control studyModerate-low
4Case seriesLow
5Expert opinionLowest

Common Biases in Clinical Research

BiasDefinitionPrevention
SelectionNon-random group allocationRandomisation, allocation concealment
PerformanceDifferential treatment of groupsBlinding
DetectionDifferential outcome assessmentBlinding of assessors
AttritionDifferential loss to follow-upITT analysis, minimise dropouts
ReportingSelective outcome reportingPre-registration, CONSORT
PublicationPositive results preferentially publishedTrial registries, funnel plots